LOCUS       Custom_genetargeter_ex 19575 bp ds-DNA     linear       14-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    10307..10352
                     /label="SEQ 1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    10361..10380
                     /label="LHR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    10381..10400
                     /label="Recoded Region"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             10410..10433
                     /label="FLAG tag"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    10443..10475
                     /label="MYC tag"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             10483..10509
                     /label="HA tag"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             10514..10537
                     /label="FLAG tag"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    10544..11403
                     /label="10x TetR aptamer array"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      11437..12272
                     /label="PfHSP86 3'UTR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      complement(12278..12841)
                     /label="PfHRP2 3'UTR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(12278..17164)
                     /label="TETR-DOZI cassette"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(12872..13267)
                     /label="BSD"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(13274..13336)
                     /label="T2as"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(13337..14269)
                     /label="Renilla luciferase (RLuc)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    14270..14332
                     /label="T2A"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(14270..14332)
                     /label="T2a"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(14339..15634)
                     /label="DOZI (DDX6 helicase)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(15641..16288)
                     /label="TetR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(16295..17164)
                     /label="PfHSP86 5'UTR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_recomb     17173..17206
                     /label="LoxP"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    17177..17448
                     /label="sgRNA Cassette"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    17215..17243
                     /label="I-CeuI\cut\site"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        17244..17260
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    17263..17282
                     /label="sgRNA Sequence"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    17283..17358
                     /label="Full gRNA sequence"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      17359..17405
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    17406..17448
                     /label="RHR_vector overlap_left"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    17449..17468
                     /label="RHR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    17469..17486
                     /label="I-SceI"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    17469..17531
                     /label="RHR_vector overlap_right"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    17487..17531
                     /label="SEQ 3"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 gcgtataatg gactattgtg tgctgataag gagaacataa gcgcagaaca atatgtatct
       61 attccggtgt tgtgttcctt tgttattctg ctattatgtt ctcttatagt gtgacgaaag
      121 cagcataatt aatcgtcact tgttctttga ttgtgttacg atatccagag acttagaaac
      181 gggggaaccg ggatgagcaa ggtaaaaatc ggtgagttga tcaacacgct tgtgaatgag
      241 gtagaggcaa ttgatgcctc agaccgccca caaggcgaca aaacgaagag aattaaagcc
      301 gcagccgcac ggtataagaa cgcgttattt aatgataaaa gaaagttccg tgggaaagga
      361 ttgcagaaaa gaataaccgc gaatactttt aacgcctata tgagcagggc aagaaagcgg
      421 tttgatgata aattacatca tagctttgat aaaaatatta ataaattatc ggaaaagtat
      481 cctctttaca gcgaagaatt atcttcatgg ctttctatgc ctacggctaa tattcgccag
      541 cacatgtcat cgttacaatc taaattgaaa gaaataatgc cgcttgccga agagttatca
      601 aatgtaagaa taggctctaa aggcagtgat gcaaaaatag caagactaat aaaaaaatat
      661 ccagattgga gttttgctct tagtgattta aacagtgatg attggaagga gcgccgtgac
      721 tatctttata agttattcca acaaggctct gcgttgttag aagaactaca ccagctcaag
      781 gtcaaccatg aggttctgta ccatctgcag ctaagccctg cggagcgtac atctatacag
      841 caacgatggg ccgatgttct gcgcgagaag aagcgtaatg ttgtggttat tgactaccca
      901 acatacatgc agtctatcta tgatattttg aataatcctg cgactttatt tagtttaaac
      961 actcgttctg gaatggcacc tttggccttt gctctggctg cggtatcagg gcgaagaatg
     1021 attgagataa tgtttcaggg tgaatttgcc gtttcaggaa agtatacggt taatttctca
     1081 gggcaagcta aaaaacgctc tgaagataaa agcgtaacca gaacgattta tactttatgc
     1141 gaagcaaaat tattcgttga attattaaca gaattgcgtt cttgctctgc tgcatctgat
     1201 ttcgatgagg ttgttaaagg atatggaaag gatgatacaa ggtctgagaa cggcaggata
     1261 aatgctattt tagcaaaagc atttaaccct tgggttaaat catttttcgg cgatgaccgt
     1321 cgtgtttata aagatagccg cgctatttac gctcgcatcg cttatgagat gttcttccgc
     1381 gtcgatccac ggtggaaaaa cgtcgacgag gatgtgttct tcatggagat tctcggacac
     1441 gacgatgaga acacccagct gcactataag cagttcaagc tggccaactt ctccagaacc
     1501 tggcgacctg aagttgggga tgaaaacacc aggctggtgg ctctgcagaa actggacgat
     1561 gaaatgccag gctttgccag aggtgacgct ggcgtccgtc tccatgaaac cgttaagcag
     1621 ctggtggagc aggacccatc agcaaaaata accaacagca ctctccgggc ctttaaattt
     1681 agcccgacga tgattagccg gtacctggag tttgccgctg atgcattggg gcagttcgtt
     1741 ggcgagaacg ggcagtggca gctgaagata gagacacctg caatcgtcct gcctgatgaa
     1801 gaatccgttg agaccatcga cgaaccggat gatgagtccc aagacgacga gctggatgaa
     1861 gatgaaattg agctcgacga gggtggcggc gatgaaccaa ccgaagagga agggccagaa
     1921 gaacatcagc caactgctct aaaacccgtc ttcaagcctg caaaaaataa cggggacgga
     1981 acgtacaaga tagagtttga atacgatgga aagcattatg cctggtccgg ccccgccgat
     2041 agccctatgg ccgcaatgcg atccgcatgg gaaacgtact acagctaaaa gaaaagccac
     2101 cggtgttaat cggtggcttt tttattgagg cctgtcccta cccatcccct gcaagggacg
     2161 gaaggattag gcggaaactg cagctgcaac tacggacatc gccgtcccga ctgcagggac
     2221 ttccccgcgt aaagcggggc ttaaattcgg gctggccaac cctatttttc tgcaatcgct
     2281 ggcgatgtta gtttcgtgga tagcgtttcc agcttttcaa tggccagctc aaaatgtgct
     2341 ggcagcacct tctccagttc cgtatcaata tcggtgatcg gcagctctcc acaagacata
     2401 ctccggcgac cgccacgaac tacatcgcgc agcagctccc gttcgtagac acgcatgttg
     2461 cccagagccg tttctgcagc cgttaatatc cggcgcagct cggcgatgat tgccgggaga
     2521 tcatccacgg ttattgggtt cggtgatggg ttcctgcagg cgcggcggag agccatccag
     2581 acgccgctaa cccatgcgtt acggtactga aaactttgtg ctatgtcgtt tatcaggccc
     2641 cgaagttctt ctttctgccg ccagtccagt ggttcaccgg cgttcttagg ctcaggctcg
     2701 acaaaagcat actcgccgtt tttccggata gctggcagaa cctcgttcgt cacccacttg
     2761 cggaaccgcc aggctgtcgt cccctgtttc accgcgtcgc ggcagcggag gattatggtg
     2821 tagagaccag attccgatac cacatttact tccctggcca tccgatcaag tttttgtgcc
     2881 tcggttaaac cgagggtcaa tttttcatca tgatccagct tacgcaatgc atcagaaggg
     2941 ttggctatat tcaatgcagc acagatatcc agcgccacaa accacgggtc accaccgaca
     3001 agaaccaccc gtatagggtg gctttcctga aatgaaaaga cggagagagc cttcattgcg
     3061 cctccccgga tttcagctgc tcagaaaggg acagggagca gccgcgagct tcctgcgtga
     3121 gttcgcgcgc gacctgcaga agttccgcag cttcctgcaa atacagcgtg gcctcataac
     3181 tggagatagt gcggtgagca gagcccacaa gcgcttcaac ctgcagcagg cgttcctcaa
     3241 tcgtctccag caggccctgg gcgtttaact gaatctggtt catgcgatca cctcgctgac
     3301 cgggatacgg gctgacagaa cgaggacaaa acggctggcg aactggcgac gagcttctcg
     3361 ctcggatgat gcaatggtgg aaaggcggtg gatatgggat tttttgtccg tgcggacgac
     3421 agctgcaaat ttgaatttga acatggtatg cattcctatc ttgtataggg tgctaccacc
     3481 agagttgaga atctctatag gggtggtagc ccagacaggg ttctcaacac cggtacaaga
     3541 agaaaccggc ccaaccgaag ttggccccat ctgagccacc ataattcagg tatgcgcaga
     3601 tttaacacac aaaaaaacac gctggcgcgt gttgtgcgct tcttgtcatt cggggttgag
     3661 aggcccggct gcagattttg ctgcagcggg gtaactctac cgccaaagca gaacgcacgt
     3721 caataattta ggtggatatt ttaccccgtg accagtcacg tgcacaggtg tttttatagt
     3781 ttgctttact gactgatcag aacctgatca gttattggag tccggtaatc ttattgatga
     3841 ccgcagccac cttagatgtt gtctcaaacc ccatacggcc acgaatgagc cactggaacg
     3901 gaatagtcag caggtacagc ggaacgaacc acaaacggtt cagacgctgc cagaacgtcg
     3961 catcacgacg ttccatccat tcggtattgt cgacgacctg gtaagcgtat tgtcctggcg
     4021 tttttgctgc ttccgagtag caatcctctt caccacaaag aaagttactt atctgcttcc
     4081 agttttcgaa cccttcttct ttgagccgct tttccagctc attcctccac aaaacaggca
     4141 cccatcctct gcgataaatc atgattattt gtcctttaaa taaggctgta gaactgcaaa
     4201 atcgctctcg ttcacatgct gtacgtagat gcgtagcaaa ttgccgttcc atccctgtaa
     4261 tccaccttct ttggaaagat cgtccttgac ctcacgaaga accttatcca atagccctgc
     4321 ggcacaagaa attgcctgct ctggatcagc aaattcatat tgattaatag gtgattgcca
     4381 cacaccaaaa acaggaatca tcttttcggc taaacgcctc tcctgttctt tcttaatctc
     4441 aagttgtaag cggaccagct caccatccat cattttttgt agatcatgcg ccactattca
     4501 cccccactgg ccatcagcaa ataaagcttc atactcggac accggcaggc ggcttccacg
     4561 gattgaaagg tcaagccaac cacgtccaga tgggtcagcc ttatccgatt cttcccaccg
     4621 ttctgcagct gtagcaacca ggcattctac cgccttcatg tagtcttctg tacggaacca
     4681 gccgtagtta atgccaccat cagtaactgc ccaggccatc tttttctctt cggcctcaat
     4741 agcccggatg cggttatcgc acagctcgcg acagtacttc agctgttcgt aatccagttg
     4801 cttcaggaac tctggtgtcg acgtcatagt ggcttcacct tataggcttt tagaagcgcc
     4861 ctggcttcgt ctgtgtggtc ttccatgctc ttatcgctgg caatgcagca ataaactccc
     4921 tcactatctg agaacccgtt catccgaatg atcgtgaatg gaagttcccg gccagtttta
     4981 taatcgctat agcttgtcgc gtcgtggctg accttgacca cataagggtc gtagccctcc
     5041 acgatgacaa ggcattcccg ttgttttccc attacccctc cggttatatc gccacggctt
     5101 gccgctggct tagaaacgct ttcagcagcc ttatttcgcg tactgatagc aggtccataa
     5161 attcggtcat gtacagcgag gcgaacgttc tcgcgatgct ggccactggc cacaggcgta
     5221 ccgcctccat ttcggttgct ggcaacgcgt tctccgccca cgcctccggt accgccaccg
     5281 ggatagcctc cagtgcctgg ataattactg attgtggggc gtccggaacg tgctctgttt
     5341 tggatcgagg gttaccatgt atatctatat ttagatccaa atcgcgatcc acttcgatgg
     5401 tggttttttc caccttacgt gcgtgaattg ataaaccggc ctcgcggcgc ttctccacga
     5461 tattcatgag gaactcgacc gagtccgggt caatggaacg catcgtgggg cgtgcatcgc
     5521 cgtctctggc gcgtctggtc ttactggata gccccataga ctccaggatg cctatgcaga
     5581 ggtctgcagg cgctttcttc ttgcctttct ctgtgttgaa gccgccgatg cgtaaaacgt
     5641 tgtttagcag atcgcgccgt tccggcgtga gcaggttatc tctggcgcgt ttgagggcgt
     5701 ccatgtctgc ttcaccttcc agggtttttg gatcgatacc gcagtcgcgg aagtactgct
     5761 gcagcgtcgc cgatttgagg gtgtagaaac cacgcatgcc tatctcaaca gcaggggtcg
     5821 atttcactcg gtaatcggtt atggccggga atttagcctg gaactctgcg tcggcctgtt
     5881 cccgcgtcat ggccgtagtg acgaactgct gccatcttcc ggcaacgcga taagcgtagg
     5941 taaagtgaat caacgcttct tcacggtcaa ggcgacgggc ggttatctca tccagctgca
     6001 tggtttcaaa caggcgcact tttttcaggc cgccgtcgaa atagaatttt aacgccacct
     6061 cgtcgacatc cagctgcagc tccttttcga tgtcccagcg gaccagctgg gcctgctcat
     6121 ccagggacag ggtgcgtttt tttatcaact catcgtgttc ggcctggtca ggagtatcga
     6181 cactcaggtg gcgctccata agctgctcaa agaccagttc acgggcttct ttacgtaaat
     6241 ccttaccgat gctgtttgca agcgcgtcgg tggccatagg cgcgacctga tagccatcat
     6301 catgcatgat gcaaatcatg ttgctggcat aatcatttct ggccgatgcc tcgagcgcgg
     6361 cggctttaat tttgagctgc atgaatgaag agttagccac gccgagtgaa attcggtcac
     6421 cgtcaaagac aacgtctgtc agcagcccgg agtggccagc cgtttcgagc aaggcctgcg
     6481 cgtaggcgcg tttgattttt tccggatcgg tttcacgttt accgcgaagc ttgtcgaaac
     6541 cgataatgta ttcctgagct gtacggtcgc ggcgcagcat ctggatggcg tcgctgggga
     6601 ccacttcgcc gcagaacatg ccgaaatggc ggtggaagtg tttctcctca atcgatacac
     6661 ctgaagatat cgacgggctg tagatgaggc cgtcatattt tttcaccatc actttaggct
     6721 ggttggtgaa atcgtcgact tccttctcct gtttgttttt ctggttaacg cagagaaact
     6781 ttttgtcagg gaactgtagt ctcagctgca tggtaacgtc ttcggcgaac gtcgaactgt
     6841 cggtggccag catgattcgt tcgccgcgtt gcactgcagc gataacctcg gtcatgatcc
     6901 gatttttctc ggtataaaat acgcggatag gcttgttggt ttcgcggttg cgaacgtcga
     6961 ccgggagttc aatcacgtga atttgcagcc aggcaggtag gcccagctcc tcgcgtcgct
     7021 tcatcgccag ttcagccagg tcaacaagca gatcgttggc atcggcatcc accataatgg
     7081 catgctcttc agtacgcgcc agcgcgtcga taagcgtgtt gaatacgcct accgggtttt
     7141 ccatcgcacg cccggccaga atggcacgca ggccctgtgt tgcttcatcg aagccgaaga
     7201 agtcatgctg gcgcatcagc ggttgccagc agcctttaag tatggagttg atgcaaatag
     7261 tcagcttgtt ggcatatggc gccatttcct gatagccggg atcctgataa tgcagaatgt
     7321 cggctttcgc gcctttccct tcggtcatca tttcatgcag gccgcctatc agggatacgc
     7381 ggtgcgcgac ggaaacgcca cgcgtggact gcagcatcag tggacgcagg aggcctgtcg
     7441 atttacccga ccccatcccg gcgcggacaa taacgatgcc ctgcagctgt gcggcgtatg
     7501 tcatcacctc atcggtcatc ctggaggttt caaaccgttt gtaagtgatg tgtgacgggc
     7561 gaaggttcgg gttggtgatg cgttcactga acgaacgtga tgtttgcgcg gcacggcatt
     7621 tgcgattcaa ccggcgcgta atgtgatctt taacggtacc gttataaatt tctgcgatac
     7681 ccatatcccg cagcgtgctg ctgaaaaggc gcataagttc tttcgggctg tttggtaccg
     7741 ggcatgtcag catgccaata tcaacggcgc gaagcagttc tttggcaaaa gtgcgtctgt
     7801 tcagacgcgg gagagtacgc agcttattca gcgtgatcga caacagatcg gttgcacggc
     7861 tcagatgatt tctcgttaac tggcgagcga cttccttcag ccctctcagg ctgtgcaggt
     7921 cgttaaaatc gctgcattcc agctcagggt catcctcaaa agttgggtaa acacatttga
     7981 cgccggaaaa cttctccatg atgtcgaatc cggtgcggag gcctgtgttg ccttttcctt
     8041 cagctgagga tttgcggtcg ttatcgagag cgcaagtgat ttgcgcagcc gggtacatgt
     8101 tcaccagctg ctcgacaacg tgaatcatgt tgttagcgga aaccgcaatg actaccgcgt
     8161 caaagcgttt tttcgggtcg tttctggtcg ccagccagat ggatgccccg gtggcgaaac
     8221 cctctgcagt cgcaattttt tgcgccccct gcaggtcgcc aataacaaag catgcaccga
     8281 cgaaatcacc gttagtgatg gcgctggtct ggaacttgcc accattcaga tcgatacgtt
     8341 gccagccaac aatccgcccg tcttttcttc cgtccaggtg ggacagaggt atcgccatgt
     8401 aagttgttgg tccacggctc catttcgcac tgtcgtgact ggtcacgcga cgtatatcac
     8461 aagcgccaaa tacgtcacga attccctttt ttaccgcata aggccaggag ccatcttcag
     8521 ctggcgaatg ttcccaggcg cgatggaaag ccaaccatcc aagcaggcgt tcctgctcca
     8581 tctgattgtt ttttaaatca ttaacgcgtt gttgttcagc tcggaggcgg cgtgcttcag
     8641 cctggcgctc catgcgtgca cgttcttctt ccggctgagc gaccacggtc gcaccattcc
     8701 gttgctgttc acggcgatac tccgaaaaca ggaatgaaaa gccactccag gagccagcgt
     8761 catgcgcttt ttcaacgaag ttaacgaaag gataactgat gccatccttg ctctgctcaa
     8821 ggcgtgaata gatttccaca cggcctttaa ggctcttctg cagagcttcc ggggaggaat
     8881 tattgtaggt ggtatagcgc tctacaccac cgcgcggatt gagctgaatc ttatcagcac
     8941 acgcaggcca gttgataccg gccatcttcg ccagctcagt cagctcatca cgtgccgcgt
     9001 caagcagtga aaacggatcg ctgccaaagc gctccgcgta gaattcttgt aaggtcattt
     9061 tttagccttt ccatgcgaat tagcattttt tcgggttgaa aaaatccgca ggagcagcca
     9121 caataaacgc actatctttc tgaaggacgt atctgcgtta tcgtggctac ttcctgaaaa
     9181 aggcccgagt ttgccgactc gggttttttt tcgtcttttt tcggctgcta cggtctggtt
     9241 caaccccgac aaagtataga tcggattaaa ccagaattat agtcagcaat aaaccctgtt
     9301 attgtatcat ctaccctcaa ccatgaacga tttgatcgta ccgactactt ggtgcacaaa
     9361 ttgaagatca cttttatcat ggataacccg ttgagagtta gcactatcaa ggtagtaatg
     9421 ctgctcgtca taacgggcta atcgttgaat tgtgatctcg ccgttattat cacaaaccag
     9481 tacatcctca cccggtacaa gcgtaagtga agaatcgacc aggataacgt ctcccggctg
     9541 gtagtttcgc tgaatctggt tcccgaccgt cagtgcgtaa acggtgttcc gttgactcac
     9601 gaacggcagg aatcgctctg tgttggcagg ttctccaggc tgccagtctc tatccggtcc
     9661 ggtctctgtc gtaccaataa caggaacgcg gtctggatca gattcagtgc catacagtat
     9721 ccattgcacg ggcttacgca ggcattttgc cagcgatagc ccgatctcca gcgacggcat
     9781 cacgtcgcca cgttctaagt tttggacgcc cggaagagag attcctacag cttctgccac
     9841 ttgcttcagc gtcagtttca gctctaaacg gcgtgctttc agtcgttcgc ctcgtgtttt
     9901 cataccctta atcataaatg atctctttat agctggctat aatttttata aattatacct
     9961 agctttaatt ttcacttatt gattataata atccccatga aacccgaaga acttgtgcgc
    10021 catttcggcg atgtggaaaa agcagcggtt ggcgtgggcg tgacacccgg cgcagtctat
    10081 caatggctgc aagctgggga gattccacct ctacgacaaa gcgatataga ggtccgtacc
    10141 gcgtacaaat taaagagtga tttcacctct cagcgcatgg gtaaggaagg gcataacagg
    10201 ggatcctcta gacgcagaaa ggcccacccg aaggtgagcc agtgtgatta catttgcggc
    10261 ctaactgtgg ccagtccagt tacgctggag tcactagtgc ggccgccggt ttaccgagct
    10321 cttattggtt ttcaaacttc attgactgtg ccggccggcc NNNNNNNNNN NNNNNNNNNN
    10381 NNNNNNNNNN NNNNNNNNNN gcgatcgcgg attataaaga tgatgatgat aaatgacgta
    10441 cggaacagaa actcatcagc gaagaagatc tgtgaCGTAC gGtatcctta tgacgtacct
    10501 gattatgcat gaCGATTATA AAGATGATGA TGATAAAGGG cccctagaaa ttatctagga
    10561 tccaggcaga gaaaggtcga tacggacgga atgtgatggc ctggatccaa cacaaaactc
    10621 gagaacatcc cggtaattat ctaggatcca ggcagagaaa ggtcgatacg gacggaatgt
    10681 gatggcctgg atccaacaca aaactcgaga acatcccggt aattatctag gatccaggca
    10741 gagaaaggtc gatacggacg gaatgtgatg gcctggatcc aacacaaaac tcgagaacat
    10801 cccggtaatt atctaggatc caggcagaga aaggtcgata cggacggaat gtgatggcct
    10861 ggatccaaca caaaactcga gaacatcccg gtaattatct aggatccagg cagagaaagg
    10921 tcgatacgga cggaatgtga tggcctggat ccaacacaaa actcgagaac atcccggaaa
    10981 ttatctagga tccaggcaga gaaaggtcga tacggacgga atgtgatggc ctggatccaa
    11041 cacaaaactc gagaacatcc cggtaattat ctaggatcca ggcagagaaa ggtcgatacg
    11101 gacggaatgt gatggcctgg atccaacaca aaactcgaga acatcccggt aattatctag
    11161 gatccaggca gagaaaggtc gatacggacg gaatgtgatg gcctggatcc aacacaaaac
    11221 tcgagaacat cccggtaatt atctaggatc caggcagaga aaggtcgata cggacggaat
    11281 gtgatggcct ggatccaaca caaaactcga gaacatcccg gtaattatct aggatccagg
    11341 cagagaaagg tcgatacgga cggaatgtga tggcctggat ccaacacaaa actcgagaac
    11401 atcccgggag atcttggcca gttaacctag tctagtttat ataatatatt tatgtactca
    11461 caatggggtc tacaaaaaaa aaaaaataaa gatatatata tatatatatt atctttcatt
    11521 ttatcatcac cccaatatac atatatatat ttttatattt ttatatattt atatttttct
    11581 tttatttatc cataaaagaa aattaaatat aaatttatac cttttatttg aattaccatt
    11641 tgtataaaat ataaaaaaaa aaaaaaataa taaaaagaag aaaaaacgaa aaaagtaata
    11701 tttaaataat aaagatcaat atacataata aaaaaaaaaa aaaaaaaaaa aattaaaatt
    11761 taaaaaatta aacattgaaa taatttcata tacacacata tacatacata catatatata
    11821 tatatatata tatatttata catttatgca tataaaatat attaacaact tatgtgtatt
    11881 catatatatt tttttacata tgtattgaaa gactttactg agacatgaaa tatctaataa
    11941 ccaaacgttt ataaattcta attatatgta aagcataata tagcttttgt ctaatttaat
    12001 tccttaaaac gtatgtatta aaaatatagt aagatgggga aattataatt ttttttttaa
    12061 aggtttttct ttgatacagt aaaaaaaaaa aataaaaaaa aataataata ataaaaaaat
    12121 aaaaaaataa aaaaaaataa aataattata aagatatata taattcctcc caaatacaaa
    12181 tgattaagtt taaaaattaa taaataaatc aaaaaaaaat ttaataataa ttgtattatt
    12241 ttaagtgtag ttaattcatc aaatagcatg cctgcagggt acctctagat gtaataaata
    12301 tgttcttata tataatgaga aataaatatt taacatatgt ttaaaaagaa aaatttaaga
    12361 tttacatgat taggaataaa aatattaaaa gctatatatt aatgttgtac tttatgttca
    12421 tattgtaaaa gataaatata atttttatta aaatttatat tattattatt ataagttctt
    12481 agaaaataca ttttgtttta aaataatact ataaaaagaa caataataaa aataaaataa
    12541 atacaattaa aacataatta aaaaagaaaa tacagacagt aaaaaaaatc gctatcccat
    12601 aaattacaaa acatgaatta ataaacatat attgtttatt aataaaattg taagttattt
    12661 ttatatatac ataataaatt aaagatactg aaacaataat tttaaatgta aataaatgaa
    12721 taatgatatg ttttatatga ttcattattc tatatttata aggaagatta caaaaaaaaa
    12781 attcatatgt attttttttg taatttctgt gtttatgttt ttatttttat aatattttta
    12841 atctattatt aaataaattt aatggaccgg tttagccctc ccacacataa ccagagggca
    12901 gcaattcacg aatcccaact gccgtcggct gtccatcact gtccttcact atggctttga
    12961 tcccaggatg cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc aagatgcccc
    13021 tgttctcatt tccgatcgcg acgatacaag tcaggttgcc agctgccgca gcagcagcag
    13081 tgcccagcac cacgagttct gcacaaggtc ccccagtaaa atgatataca ttgacaccag
    13141 tgaagatgcg gccgtcgcta gagagagctg cgctggcgac gctgtagtct tcagagatgg
    13201 ggatgctgtt gattgtagcc gttgctcttt caatgagggt ggattcttct tgagacaaag
    13261 gcttggcgcc ggctggtcct ggattttctt ctacatctcc acatgttaat aaacttcctc
    13321 ttccttctcc acttccttgt tcatttttga gaactcgctc aacgaacgat ttgatatatt
    13381 ttcccatttc atcaggtgca tcttcttgcg aaaaatgaag accttttact ttgacaaatt
    13441 cagtattagg aaacttcttg gcaccttcaa caatagcatt ggaaaagaat cctgggtccg
    13501 attcaataaa catttttggt aaatcatcac ttgcacgtag ataagcatta taattcctaa
    13561 caatttgtac aacgtcaggt ttaccacctt ttactaacgg gatttcacga ggccatgata
    13621 atgttggacg acgaacttca cctttctctt tgaatggttc aagatatgct gcaaattctt
    13681 ctggttctaa ctttctcatg atttttgatg gcaacatggt ttccacgaag aagttattct
    13741 ccaaaaccat tttttctcct tcttcagatt tgatcaacgc aatatcttct tcaatatcag
    13801 gccattcatc ccatgattca atcacatcta ctacactttc agcgtgaact attgctttga
    13861 tcttatcttg atgctcatag ctataatgaa atgccaaaca agcaccccaa tcatggccga
    13921 caaaaatgat cttctttggt aaattaagaa gttcaaacca tgcagtaaga tatttgtaat
    13981 gatcaagtaa cctataagaa ccattaccag atttgcctga tttgcccata ccaataaggt
    14041 ctggtataat acaccgcgct actggctcaa tatgtggcac aacatgtcgc cataaataag
    14101 aagaggccgc gttaccatgt aaaaaaataa cagcattttc tgcatgtttt tctgaatcat
    14161 aataattaat aaatgaatca agaacattca tttgtttaca tctggcccac cactgcggac
    14221 cagttatcat ccgtttcctt tgttctggat cataaacttt cgaagtcatt ggacctggat
    14281 tttcttcaac atcaccacat gttaataatg atcctctacc ttcaccacta ccgctagcgg
    14341 tatataagga tgggtcaatt tcgtttggta ttggttgtat ttccgttcct agttctactt
    14401 ctattttata taaattaaaa cgatcttcaa aagttataag attaatagct agtcctagat
    14461 gtccgtatct tcctgatctt cctattctat gtaaataagt ttcagaattc tttgggaaat
    14521 caaaattgat aacaacattg actgactgta tgtcgatacc tcttgtgaat agatctgatg
    14581 aaactaaaca tctgcatgct ccatttctaa aatcatgaaa aacacgatta cgatgtgttt
    14641 gtgacattct tgcatgaatg taaaaagagc tatatcctag ttcggtaatt tttttggcta
    14701 gtagttctac cctagtaata ctattacaga agatgatagc ttgattaatt tgaagtttag
    14761 caaataatgt atttaaacaa tgtacttttt gtctttcttt aacaaaagca taatattgtg
    14821 ttattccttt taaggttaat tcatccataa gatttatttc atgggcatct gataaataaa
    14881 tagctcgaaa ttcttttaca gtcacaggaa aggtagcaga atacataagt atctgctttt
    14941 cttttggtaa aaatttcatt agttcttcta ctataggttg aaattcaggt gataataatt
    15001 tatctgcttc atccataacc ataatatgac aacctgataa atttgctaca tccttatttg
    15061 ctaagtctaa tattcttcct ggagtaccac ataaaatatg aactacatta tacaacctca
    15121 ttatatcttc tcttaatgat gtaccaccgg ttgttaccat acactgtact ttcatgtgtt
    15181 ttcctaattc cttaatcata gcagaggtct gtagggcaag ttctcgcgtg ggtaccaaaa
    15241 tgagtccttg aataaaattt ttgtgggtat tacatttctc tagtaagggt atagcaaaag
    15301 ctgctgtttt gcctgtacca ttttttgccc ttgctaaaat attttttcca gccaaagcta
    15361 caggtatact ttcctcttgt ataggtgatg gtttctcata tcctttttca aagataccca
    15421 ttaataattc tctcttcaaa aaataatctt caaattcatt gcctttcgtt ttcgttacat
    15481 cttctgtctt atatcttaga tcttttaatg gttccagaat tttctttttc cattcttcat
    15541 ctaatattat attatcatct attttgttat aatttgaaga actattcaaa gtatttgtat
    15601 tagcattaga gttcgtacaa ttggttttat aactactagt accaccacca ccaccaccac
    15661 caccaccaga cccactttca catttaagtt gtttttctaa tccgcatatg atcaattcaa
    15721 ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa taattcgata gcttgtcgta
    15781 ataatggcgg catactatca gtagtaggtg tttccctttc ttctttagcg acttgatgct
    15841 cttgatcttc caatacgcaa cctaaagtaa aatgccccac agcgctgagt gcatataatg
    15901 cattctctag tgaaaaacct tgttggcata aaaaggctaa ttgattttcg agagtttcat
    15961 actgtttttc tgtaggccgt gtacctaaat gtacttttgc tccatcgcga tgacttagta
    16021 aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt tccccttcta
    16081 aagggcaaaa gtgagtatgg tgcctatcta acatctcaat ggctaaggcg tcgagcaaag
    16141 cccgcttatt ttttacatgc caatacaatg taggctgctc tacacctagc ttctgggcga
    16201 gtttacgggt tgttaaacct tcgattccga cctcattaag cagctctaat gcgctgttaa
    16261 tcactttact tttatctaat ctagacatcc taggtttatt cgaaatgtgg gaagaaaaaa
    16321 aatataataa aaaaggaaaa tttcttttta aagtaaaata tttatgtaaa tatttatata
    16381 ttttattata tatatattat tatatatata tatatatata tatataccaa taaaataata
    16441 taaaaatttt tatgtatatc taaaaatatg tttttattaa aaggtaaaaa taaacttatt
    16501 ttttcttttt ttttaaaaaa tatatatata tatatatata tatatataat attatatgag
    16561 atatatatta aatattatat tatttatata aatataattt ttttcttaat ttctcacgtt
    16621 gttaaaattt atatatttat atatgatata tattttttaa tactttttaa ttttttttat
    16681 tatttttggt aattatctta ttttatttta attttattat tttatattaa atgatgcttt
    16741 ttattttata ttattatttt ttatttaaaa tttttgctaa acatgaaatt attttattat
    16801 attttgtaaa tacaaaataa aataatatat aaatcaaaaa aaaaaaaaaa gaaagaaaga
    16861 aagaaagaaa gaaagaaaca aagatgatat ttaaaaaaaa aaagatatta taaaaatata
    16921 atattatata taaaatatta tttttatata aatatattta tatatttata tatttatata
    16981 tatgtatata tatatatata tatatatagg tatagtattt attattttgt tatatttata
    17041 taatactcct ttttgttatt tttttttttt ttttctttaa attcatgcaa aaatttacta
    17101 taatattatt atatttaact atatactatg gaatactaaa tatatatcca atggcccctt
    17161 tccgggcgcg ccataacttc gtatagcata cattatacga agttatGGCG CGCCcgtaac
    17221 tataacggtc ctaaggtagc gaataatacg actcactata ggNNNNNNNN NNNNNNNNNN
    17281 NNgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
    17341 gtggcaccga gtcggtgcta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
    17401 ttttgcggtc tcagtggtgt acggtacaaa cccggaattc gagctcggNN NNNNNNNNNN
    17461 NNNNNNNNat taccctgtta tccctaggtc taatacccaa tctctcgtct tatccagatg
    17521 ttttatacgc cgcggccgct tacaactgga ccttgctggt acatagaact gattaactga
    17581 ccatttaaat cataccaaca tggtcaaata aaacgaaagg ctcagtcgaa agactgggcc
    17641 tttcgtttta atctgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
    17701 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg
    17761 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
    17821 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
    17881 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
    17941 ttcttgcccg cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc
    18001 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
    18061 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
    18121 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
    18181 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
    18241 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
    18301 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
    18361 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaccta
    18421 ggtgacagaa gtcaaaagcc tccggtcgga ggcttttgac tttctgctag atctgtttca
    18481 atgcggtgaa gggccaggca gctggggatt atgtcgagac ccggccagca tgttggtttt
    18541 atcgcatatt cagcgttgtc gcgtttaccc aggtaaaatg gaagcagtgt atcgtctgcg
    18601 tgaatgtgca aatcaggaac gtaaccgtgg tacatagatg cagtcccttg cgggtcgttc
    18661 ccttcaacga gtaggacgcg gtgcccttgc aaggctaacc attgcgcctg gtgtactgca
    18721 gatgaggttt tataaacccc tcccttgtgt gacataacgg aaagtacaac cgggttttta
    18781 tcgtcaggtc tttggtttgg gttaccaaac acactccgca tatggctaat ttggtcaatt
    18841 gtgtagccag cgcgacgttc tactcggccc ctcatctcaa aatcaggagc cggtagacga
    18901 ccagcttttt ccgcgtctct gatagcctgc ggtgttacgc cgatcaggtc tgcaacttct
    18961 gttatacccc agcggcgagt aatacgacgc gcttccgggc tgtcatcgcc gaactgtgcg
    19021 atggcaatag cgcgcgtcat ttcctgaccg cgattgatac agtctttcag caaattaatt
    19081 aacgacatcc tgtttcctct caaacatgcc cttatctttg tgtttttcat catactttac
    19141 gtttttaaag caaagcaaca taaaaaaagc aaagtgactt agaaaacgca aagttaaggt
    19201 tcaaatcaat tttttgatgc gctacagaag ctatttagct tcatctaagc gcaacggtat
    19261 tacttacgtt ggtatattta aaacctaact taatgatttt aaatgataat aaatcatacc
    19321 aattgctatc aaaagttaag cgaacatgct gattttcacg ctgtttatac actttgaggc
    19381 atctctatct cttccgtctc tatattgaaa cacaatcaaa gaacatcaat ccatgtgaca
    19441 tcccccacta tctaagaaca ccataacaga acacaacata ggaatgcaac attaatgtat
    19501 caataattcg gaacatatgc actatatcat atctcaatta cggaacatat cagcacacaa
    19561 ttgcccatta tacgc
//